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My primary technique is 3 Dimensional Microscopy using Ultraviolet Excitation (3D-MUSE). This technique produces volumetric, high resolution histological data with a large field of view, 9.36 x 7.02 mm, allowing for us to create 3D models of tissues. This 3D-MUSE system provides several advantages.

First, the limited tissue penetration depth of UV light at 280 nm provides optical sectioning by confining fluorescence excitation and absorption to the tissue surface, reducing subsurface fluorescence that can degrade effective resolution and contrast.

Second, a wide range of commonly used MUSE dyes are fluorescent in the visible range, allowing image acquisition with a color camera.

Third, UV light in the appropriate spectral ranges can be generated with inexpensive LED sources. As UV light below 300 nm is absorbed by glass, the microscope optics intrinsically block stray excitation light, thus eliminating the need for specific excitation and emission filter sets.

Finally, the process of creating images of the tissue surface with this technique is almost instantaneous, providing a significant time savings compared to conventional histology.